Difference between revisions of "Part:BBa K1642011:Design"
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<partinfo>BBa_K1642011 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1642011 SequenceAndFeatures</partinfo> | ||
− | + | [[File:SJTUB wb Pdark-HR.jpeg|200px] | |
===Design Notes=== | ===Design Notes=== |
Revision as of 16:14, 18 September 2015
Expression of Halorhodopsin under the control of Pdrak
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 611
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3215
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 481
Illegal NgoMIV site found at 783
Illegal NgoMIV site found at 942
Illegal NgoMIV site found at 1263
Illegal AgeI site found at 1134 - 1000COMPATIBLE WITH RFC[1000]
[[File:SJTUB wb Pdark-HR.jpeg|200px]
Design Notes
Pdark(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Control_System)
HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)
Pdark-HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Design)
Source
Overlap PCR from basic parts Up(BBa_K1642005), Pdark(BBa_K1026009), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006).