Difference between revisions of "Part:BBa K1593208"

 
 
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====Description====
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SoxS, a transcriptional activator of oxidative stress genes in E. coli. SoxS in vitro binds the promoter of soxRS-regulated genes such as micF, sodA and recruits RNA polymerase to the promoters.
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SoxS was originally tested by Sahar Melamed and Shimshon Belkin to research the antibiotic resistance of bacteria and features of selected sensing promoters. Sensitivity and specificity of SoxS was presented in their paper, showing its relatively specific signal response when treated with:
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*'''tetracycline'''-26.5;
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*'''oxytetracycline'''-26.1.
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The maximal induction of SoxS strains tested in the course of 10h of exposure.
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[[Image: Promoter_Selection_with_Fluroescence_Intensity.png|thumb|none|500px|Test Result]]
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====Application====
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In accordance with relative effective response of antibiotics, we are determined to implement SoxS to detect the concentration of tetracycline or oxytetracycline.
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[[Image:Soxs.png|thumb|none|500px|Sensing circuit using SoxS]]
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====Experimental Result====
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SoxS, this antibiotic substance responding promoter was extracted from E. coli Top 10(K-12 strain) by conducting polymerase chain reaction on its genome.
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[[Image:MicF_and_SoxS.png|thumb|none|500px| Electrophoretogram of micF(right)]]
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====Reference====
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<html><i>A bacterial reporter panel for the detection and classification of antibiotic substances <b>Microbial Biotechnology (2012) 5(4), 536–548</b> Sahar Melamed, Shimshon Belkin</i><html>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1593208 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1593208 SequenceAndFeatures</partinfo>

Latest revision as of 16:11, 18 September 2015

soxS Promoter, Sensing Antibiotics

This is a promoter sensing tetracycline which is previously selected from by Sahar Melamed and Shimshon Belkin

Description

SoxS, a transcriptional activator of oxidative stress genes in E. coli. SoxS in vitro binds the promoter of soxRS-regulated genes such as micF, sodA and recruits RNA polymerase to the promoters. SoxS was originally tested by Sahar Melamed and Shimshon Belkin to research the antibiotic resistance of bacteria and features of selected sensing promoters. Sensitivity and specificity of SoxS was presented in their paper, showing its relatively specific signal response when treated with:

  • tetracycline-26.5;
  • oxytetracycline-26.1.

The maximal induction of SoxS strains tested in the course of 10h of exposure.

Test Result

Application

In accordance with relative effective response of antibiotics, we are determined to implement SoxS to detect the concentration of tetracycline or oxytetracycline.

Sensing circuit using SoxS

Experimental Result

SoxS, this antibiotic substance responding promoter was extracted from E. coli Top 10(K-12 strain) by conducting polymerase chain reaction on its genome.

Electrophoretogram of micF(right)

Reference

A bacterial reporter panel for the detection and classification of antibiotic substances Microbial Biotechnology (2012) 5(4), 536–548 Sahar Melamed, Shimshon Belkin Sequence and Features BBa_K1593208 SequenceAndFeatures