Difference between revisions of "Part:BBa K1725301"
Line 12: Line 12:
 
| Degenerate sequence
 
| Degenerate sequence
 
| TCACACA<font color="red">NR</font>A<font color="red">RR</font>G
 
| TCACACA<font color="red">NR</font>A<font color="red">RR</font>G
−
| row 1, cell 3
+
|  
 
|-
 
|-
 
| BBa_K1725301
 
| BBa_K1725301
 
|  TCACACA<font color="red">GG</font>A<font color="red">GG</font>G
 
|  TCACACA<font color="red">GG</font>A<font color="red">GG</font>G
−
| row 1, cell 3
+
| 15730.86
 
|-
 
|-
 
| BBa_K1725302
 
| BBa_K1725302
 
|  TCACACA<font color="red">GG</font>A<font color="red">GA</font>G
 
|  TCACACA<font color="red">GG</font>A<font color="red">GA</font>G
−
| row 2, cell 3
+
| 12493.41
 
|-
 
|-
 
| BBa_K1725303
 
| BBa_K1725303
 
|  TCACACA<font color="red">GG</font>A<font color="red">AG</font>G
 
|  TCACACA<font color="red">GG</font>A<font color="red">AG</font>G
−
| row 1, cell 3
+
| 8716.05
 
|-
 
|-
 
| BBa_K1725304
 
| BBa_K1725304
 
|  TCACACA<font color="red">AG</font>A<font color="red">GG</font>G
 
|  TCACACA<font color="red">AG</font>A<font color="red">GG</font>G
−
| row 1, cell 3
+
| 7247.43
 
|-
 
|-
 
| BBa_K1725305
 
| BBa_K1725305
 
|  TCACACA<font color="red">AG</font>A<font color="red">AG</font>G
 
|  TCACACA<font color="red">AG</font>A<font color="red">AG</font>G
−
| row 2, cell 3
+
| 5532.39
 
|-
 
|-
 
| BBa_K1725306
 
| BBa_K1725306
 
|  TCACACA<font color="red">CG</font>A<font color="red">GG</font>G
 
|  TCACACA<font color="red">CG</font>A<font color="red">GG</font>G
−
| row 1, cell 3
+
| 3880.58
 
|-
 
|-
 
| BBa_K1725307
 
| BBa_K1725307
 
|  TCACACA<font color="red">TG</font>A<font color="red">GG</font>G
 
|  TCACACA<font color="red">TG</font>A<font color="red">GG</font>G
−
| row 1, cell 3
+
| 3880.58
 
|-
 
|-
 
| BBa_K1725308
 
| BBa_K1725308
 
| TCACACA<font color="red">AA</font>A<font color="red">AG</font>G
 
| TCACACA<font color="red">AA</font>A<font color="red">AG</font>G
−
| row 2, cell 3
+
| 2574.21
 
|-
 
|-
 
| BBa_K1725309
 
| BBa_K1725309
 
|  TCACACA<font color="red">AA</font>A<font color="red">GG</font>G
 
|  TCACACA<font color="red">AA</font>A<font color="red">GG</font>G
−
| row 1, cell 3
+
| 2574.21
 
|-
 
|-
 
| BBa_K1725310
 
| BBa_K1725310
 
|  TCACACA<font color="red">CA</font>A<font color="red">AG</font>G
 
|  TCACACA<font color="red">CA</font>A<font color="red">AG</font>G
−
| row 1, cell 3
+
| 2574.21
 
|-
 
|-
 
| BBa_K1725311
 
| BBa_K1725311
 
|  TCACACA<font color="red">CA</font>A<font color="red">GG</font>G
 
|  TCACACA<font color="red">CA</font>A<font color="red">GG</font>G
−
| row 2, cell 3
+
| 2574.21
 
|-
 
|-
 
| BBa_K1725312
 
| BBa_K1725312
 
|  TCACACA<font color="red">GA</font>A<font color="red">AG</font>G
 
|  TCACACA<font color="red">GA</font>A<font color="red">AG</font>G
−
| row 1, cell 3
+
| 2574.21
 
|-
 
|-
 
| BBa_K1725313
 
| BBa_K1725313
 
|  TCACACA<font color="red">TA</font>A<font color="red">AG</font>G
 
|  TCACACA<font color="red">TA</font>A<font color="red">AG</font>G
−
| row 1, cell 3
+
| 2574.21
 
|-
 
|-
 
| BBa_K1725314
 
| BBa_K1725314
 
|  TCACACA<font color="red">TA</font>A<font color="red">GG</font>G
 
|  TCACACA<font color="red">TA</font>A<font color="red">GG</font>G
−
| row 2, cell 3
+
| 2574.21
 
|-
 
|-
 
| BBa_K1725315
 
| BBa_K1725315
 
|  TCACACA<font color="red">TG</font>A<font color="red">AG</font>G
 
|  TCACACA<font color="red">TG</font>A<font color="red">AG</font>G
−
| row 1, cell 3
+
| 2574.21
 
|-
 
|-
 
| BBa_K1725316
 
| BBa_K1725316
 
|  TCACACA<font color="red">CG</font>A<font color="red">AG</font>G
 
|  TCACACA<font color="red">CG</font>A<font color="red">AG</font>G
−
| row 1, cell 3
+
| 2352.63
 
|-
 
|-
 
| BBa_K1725317
 
| BBa_K1725317
 
|  TCACACA<font color="red">GG</font>A<font color="red">AA</font>G
 
|  TCACACA<font color="red">GG</font>A<font color="red">AA</font>G
−
| row 2, cell 3
+
| 2161.77
 
|-
 
|-
 
| BBa_K1725318
 
| BBa_K1725318
 
|  TCACACA<font color="red">AG</font>A<font color="red">GA</font>G
 
|  TCACACA<font color="red">AG</font>A<font color="red">GA</font>G
−
| row 1, cell 3
+
| 1969.19
 
|-
 
|-
 
| BBa_K1725319
 
| BBa_K1725319
 
|  TCACACA<font color="red">TG</font>A<font color="red">GA</font>G
 
|  TCACACA<font color="red">TG</font>A<font color="red">GA</font>G
−
| row 1, cell 3
+
| 1506.8
 
|-
 
|-
 
| BBa_K1725320
 
| BBa_K1725320
 
|  TCACACA<font color="red">CG</font>A<font color="red">GA</font>G
 
|  TCACACA<font color="red">CG</font>A<font color="red">GA</font>G
−
| row 2, cell 3
+
| 1500.04
 
|-
 
|-
 
| BBa_K1725321
 
| BBa_K1725321
 
|  TCACACA<font color="red">GA</font>A<font color="red">GG</font>G
 
|  TCACACA<font color="red">GA</font>A<font color="red">GG</font>G
−
| row 1, cell 3
+
| 1203.18
 
|-
 
|-
 
| BBa_K1725322
 
| BBa_K1725322
 
|  TCACACA<font color="red">AA</font>A<font color="red">GA</font>G
 
|  TCACACA<font color="red">AA</font>A<font color="red">GA</font>G
−
| row 1, cell 3
+
| 875.94
 
|-
 
|-
 
| BBa_K1725323
 
| BBa_K1725323
 
| TCACACA<font color="red">CA</font>A<font color="red">GA</font>G
 
| TCACACA<font color="red">CA</font>A<font color="red">GA</font>G
−
| row 2, cell 3
+
| 875.94
 
|-
 
|-
 
| BBa_K1725324
 
| BBa_K1725324
 
|  TCACACA<font color="red">GA</font>A<font color="red">GA</font>G
 
|  TCACACA<font color="red">GA</font>A<font color="red">GA</font>G
−
| row 1, cell 3
+
| 875.94
 
|-
 
|-
 
| BBa_K1725325
 
| BBa_K1725325
 
|  TCACACA<font color="red">TA</font>A<font color="red">GA</font>G
 
|  TCACACA<font color="red">TA</font>A<font color="red">GA</font>G
−
| row 1, cell 3
+
| 875.94
 
|-
 
|-
 
| BBa_K1725326
 
| BBa_K1725326
 
|  TCACACA<font color="red">AG</font>A<font color="red">AA</font>G
 
|  TCACACA<font color="red">AG</font>A<font color="red">AA</font>G
−
| row 2, cell 3
+
| 355.35
 
|-
 
|-
 
| BBa_K1725327
 
| BBa_K1725327
 
|  TCACACA<font color="red">AA</font>A<font color="red">AA</font>G
 
|  TCACACA<font color="red">AA</font>A<font color="red">AA</font>G
−
| row 1, cell 3
+
| 271.26
 
|-
 
|-
 
| BBa_K1725328
 
| BBa_K1725328
 
|  TCACACA<font color="red">CA</font>A<font color="red">AA</font>G
 
|  TCACACA<font color="red">CA</font>A<font color="red">AA</font>G
−
| row 1, cell 3
+
| 271.26
 
|-
 
|-
 
| BBa_K1725329
 
| BBa_K1725329
 
|  TCACACA<font color="red">GA</font>A<font color="red">AA</font>G
 
|  TCACACA<font color="red">GA</font>A<font color="red">AA</font>G
−
| row 2, cell 3
+
| 271.26
 
|-
 
|-
 
| BBa_K1725330
 
| BBa_K1725330
 
|  TCACACA<font color="red">TA</font>A<font color="red">AA</font>G
 
|  TCACACA<font color="red">TA</font>A<font color="red">AA</font>G
−
| row 1, cell 3
+
| 271.26
 
|-
 
|-
 
| BBa_K1725331
 
| BBa_K1725331
 
|  TCACACA<font color="red">TG</font>A<font color="red">AA</font>G
 
|  TCACACA<font color="red">TG</font>A<font color="red">AA</font>G
−
| row 1, cell 3
+
| 271.26
 
|-
 
|-
 
| BBa_K1725332
 
| BBa_K1725332
 
|  TCACACA<font color="red">CG</font>A<font color="red">AA</font>G
 
|  TCACACA<font color="red">CG</font>A<font color="red">AA</font>G
−
| row 2, cell 3
+
| 158.07
 
|}
 
|}
  

Revision as of 15:46, 18 September 2015

Member of the RBS library - derived from BBa_B0032

Parts K1725301-K1725332 are members of ribosome binding site library derived from Anderson-family RBS B0032 (BBa_B0032). All 32 sequences were obtained by randomised PCR.

Identifier Sequence Estimated strength (au)
Degenerate sequence TCACACANRARRG
BBa_K1725301 TCACACAGGAGGG 15730.86
BBa_K1725302 TCACACAGGAGAG 12493.41
BBa_K1725303 TCACACAGGAAGG 8716.05
BBa_K1725304 TCACACAAGAGGG 7247.43
BBa_K1725305 TCACACAAGAAGG 5532.39
BBa_K1725306 TCACACACGAGGG 3880.58
BBa_K1725307 TCACACATGAGGG 3880.58
BBa_K1725308 TCACACAAAAAGG 2574.21
BBa_K1725309 TCACACAAAAGGG 2574.21
BBa_K1725310 TCACACACAAAGG 2574.21
BBa_K1725311 TCACACACAAGGG 2574.21
BBa_K1725312 TCACACAGAAAGG 2574.21
BBa_K1725313 TCACACATAAAGG 2574.21
BBa_K1725314 TCACACATAAGGG 2574.21
BBa_K1725315 TCACACATGAAGG 2574.21
BBa_K1725316 TCACACACGAAGG 2352.63
BBa_K1725317 TCACACAGGAAAG 2161.77
BBa_K1725318 TCACACAAGAGAG 1969.19
BBa_K1725319 TCACACATGAGAG 1506.8
BBa_K1725320 TCACACACGAGAG 1500.04
BBa_K1725321 TCACACAGAAGGG 1203.18
BBa_K1725322 TCACACAAAAGAG 875.94
BBa_K1725323 TCACACACAAGAG 875.94
BBa_K1725324 TCACACAGAAGAG 875.94
BBa_K1725325 TCACACATAAGAG 875.94
BBa_K1725326 TCACACAAGAAAG 355.35
BBa_K1725327 TCACACAAAAAAG 271.26
BBa_K1725328 TCACACACAAAAG 271.26
BBa_K1725329 TCACACAGAAAAG 271.26
BBa_K1725330 TCACACATAAAAG 271.26
BBa_K1725331 TCACACATGAAAG 271.26
BBa_K1725332 TCACACACGAAAG 158.07


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]