Difference between revisions of "Part:BBa K1587001"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1587001 short</partinfo> | <partinfo>BBa_K1587001 short</partinfo> | ||
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<center>[[File:TLSE tesB.jpg]]</center> | <center>[[File:TLSE tesB.jpg]]</center> | ||
− | <center>'''Figure 1 | + | <center>'''Figure 1 Butyrate formation catalyzed by tesB'''</center> |
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+ | The acyl-CoA thioesterase 2 (tesB) is an enzyme of biosynthetic butyrate pathway. This enzyme is endogenous to ''Escherichia coli'', its substrate is Butyryl coA and the direct product is butyrate. It needs the coenzyme A (coA-SH) for the synthesis of butyrate (Figure 1).<br> | ||
+ | The integrity of our construct was checked by sequencing using VF2 primer. | ||
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+ | We used BBa_K1587001 with other genes to produce butyrate in ''Escherichia coli'' (Figure 2) | ||
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+ | <center>[[File:TLSE ButMetabo.jpg]]</center> | ||
+ | <center>'''Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in ''E. coli'''''</center> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 15:22, 18 September 2015
acyl-CoA thioesterase 2
The acyl-CoA thioesterase 2 (tesB) is an enzyme of biosynthetic butyrate pathway. This enzyme is endogenous to Escherichia coli, its substrate is Butyryl coA and the direct product is butyrate. It needs the coenzyme A (coA-SH) for the synthesis of butyrate (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.
We used BBa_K1587001 with other genes to produce butyrate in Escherichia coli (Figure 2)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 542
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 145
Illegal SapI.rc site found at 157