Difference between revisions of "Part:BBa K1587001"
 
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<partinfo>BBa_K1587001 short</partinfo>
 
<partinfo>BBa_K1587001 short</partinfo>
  
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The acyl-CoA thioesterase 2 (tesB) is an enzyme of biosynthetic butyrate pathway. This enzyme is endogenous to Escherichia coli bacterium, its substrate is Butyryl coA and the direct product is butyrate. It needs the coenzyme A (coA-SH) for the synthesis of butyrate.
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<center>[[File:TLSE tesB.jpg]]</center>
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<center>'''Figure 1 Butyrate formation catalyzed by tesB'''</center>
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The acyl-CoA thioesterase 2 (tesB) is an enzyme of biosynthetic butyrate pathway. This enzyme is endogenous to ''Escherichia coli'', its substrate is Butyryl coA and the direct product is butyrate. It needs the coenzyme A (coA-SH) for the synthesis of butyrate (Figure 1).<br>
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The integrity of our construct was checked by sequencing using VF2 primer.
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We used BBa_K1587001 with other genes to produce butyrate in ''Escherichia coli'' (Figure 2)
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<center>[[File:TLSE ButMetabo.jpg]]</center>
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<center>'''Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in ''E. coli'''''</center>
  
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<center>[[File:TLSE-tesB.jpg]]</center>
 
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'''Figure 1 Acetoacetyl-coA formation catalyzed by tesB'''
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:22, 18 September 2015

acyl-CoA thioesterase 2

TLSE tesB.jpg
Figure 1 Butyrate formation catalyzed by tesB

The acyl-CoA thioesterase 2 (tesB) is an enzyme of biosynthetic butyrate pathway. This enzyme is endogenous to Escherichia coli, its substrate is Butyryl coA and the direct product is butyrate. It needs the coenzyme A (coA-SH) for the synthesis of butyrate (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.

We used BBa_K1587001 with other genes to produce butyrate in Escherichia coli (Figure 2)

TLSE ButMetabo.jpg
Figure 2: Metabolic pathway to produce butyrate via glucose using BBa_K1587004 in E. coli


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 542
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 145
    Illegal SapI.rc site found at 157