Difference between revisions of "Part:BBa K1633008:Design"
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There are several Pst I and EcoR I restriction enzyme sites in original nSMase 2 gene sequence. We optimized the gene sequence codon to fulfill the requirements. | There are several Pst I and EcoR I restriction enzyme sites in original nSMase 2 gene sequence. We optimized the gene sequence codon to fulfill the requirements. | ||
| − | + | This part is a codon-optimized nSMase 2 gene CDS. We express this gene in HEK293 cell to increase the amount of exosomes. | |
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===Source=== | ===Source=== | ||
Latest revision as of 15:04, 18 September 2015
coding sequence to express nSMase 2 protein.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 472
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1609
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 278
Illegal BsaI.rc site found at 694
Illegal BsaI.rc site found at 1600
Design Notes
There are several Pst I and EcoR I restriction enzyme sites in original nSMase 2 gene sequence. We optimized the gene sequence codon to fulfill the requirements.
This part is a codon-optimized nSMase 2 gene CDS. We express this gene in HEK293 cell to increase the amount of exosomes.
Source
Homo Sapiens. The original sequence is from NCBI. NCBI Gene ID is 55512. The sequence codon of this part was optimized. We ordered the sequence from a DNA synthesis company