Difference between revisions of "Part:BBa K1789011"

Revision as of 14:44, 18 September 2015

IAAH+Ter

It is the combination of IAAH and terminatior.

Usage and Biology

This part is constructed with IaaH and a terminatior.

IaaH is the tryptophan-2-mono-oxygenase. This enzyme catalyzes the oxidative carboxylation of L-tryptophan to indole-3-acetamide. IaaH is the indoleacetimide hydrolase. This enzyme catalyses the hydrolysis of indoleacetamide to indoleacetate and ammonia. This pathway is relevantly easy to be expressed in prokaryotic cells, and its substrate (Trp) can be synthesized by host cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1005
1000
COMPATIBLE WITH RFC[1000]

Experimental Validation

Sequencing

This part is sequenced as correct after construction.

Enzyme digestion test

A double-enzyme digestion test was implemented to verify this part.

File:IAAH.jpg

The length of the digested DNA fits the design.

Functional Test

The function of this part can be verified with in the experimental validation of BBa_K1789022.

This parts functional test can be involved into k1789022.

@@ Line 14: / Line 14: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1789011 SequenceAndFeatures</partinfo>
+==Experimental Validation==
+===Sequencing===
+This part is sequenced as correct after construction.
+===Enzyme digestion test ===
+A double-enzyme digestion test was implemented to verify this part.
+[[File:IAAH.jpg|250px|]]
+The length of the digested DNA fits the design.
+===Functional Test===
+The function of this part can be verified with in the experimental validation of BBa_K1789022.
 <!-- Uncomment this to enable Functional Parameter display
@@ Line 20: / Line 35: @@
 <partinfo>BBa_K1789011 parameters</partinfo>
 <!-- -->
+This parts functional test can be involved into k1789022.