Difference between revisions of "Part:BBa K1632007"
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[[Image:Tokyo Tech fim switch(wild-type) design.png|thumb|center|400px|Fig. 1. Overview of fim inversion system]]<br>
 
[[Image:Tokyo Tech fim switch(wild-type) design.png|thumb|center|400px|Fig. 1. Overview of fim inversion system]]<br>
  
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<span style="margin-left: 10px;">''Fim'' switch (wild-type) is derived from wild-type sequence. ''Fim'' switch (wild-type) have sigma 70 promoter which is constitutive promoter. The promoter in the ''fim'' switch transcripts to the right. From the direction of transcription, the state is defined as ON. On the other hand, fim switch [default OFF](wild-type) (<partinfo>BBa_K1632005</partinfo>) transcripts to the left.<br>
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<span style="margin-left: 10px;">''Fim'' switch (wild-type) is derived from wild-type sequence. ''Fim'' switch (wild-type) have sigma 70 promoter which is constitutive promoter. The promoter in the ''fim'' switch transcripts to the right. From the direction of transcription, the state is defined as [ON] state. On the other hand, fim switch [default OFF](wild-type) (<partinfo>BBa_K1632005</partinfo>) transcripts to the left.<br>
  
 
<span style="margin-left: 10px;">''Fim'' switch is inverted by two recombinase, FimB and FimE. The FimB protein inverts ''fim'' switch in [ON] state to [OFF] state and [OFF] to [ON] state with approximately equal efficiencies. On the other hand, the FimE protein inverts ''fim'' switch predominantly [ON] state to [OFF] state<br>
 
<span style="margin-left: 10px;">''Fim'' switch is inverted by two recombinase, FimB and FimE. The FimB protein inverts ''fim'' switch in [ON] state to [OFF] state and [OFF] to [ON] state with approximately equal efficiencies. On the other hand, the FimE protein inverts ''fim'' switch predominantly [ON] state to [OFF] state<br>

Revision as of 14:40, 18 September 2015

fim switch[default ON](wild-type)_rbs_gfp

File:Tokyo Tech fim switch(wild-type) design.png
Fig. 1. Overview of fim inversion system

Fim switch (wild-type) is derived from wild-type sequence. Fim switch (wild-type) have sigma 70 promoter which is constitutive promoter. The promoter in the fim switch transcripts to the right. From the direction of transcription, the state is defined as [ON] state. On the other hand, fim switch [default OFF](wild-type) (BBa_K1632005) transcripts to the left.

Fim switch is inverted by two recombinase, FimB and FimE. The FimB protein inverts fim switch in [ON] state to [OFF] state and [OFF] to [ON] state with approximately equal efficiencies. On the other hand, the FimE protein inverts fim switch predominantly [ON] state to [OFF] state

We constructed fim switch [default ON](wild-type)_rbs_gfp(BBa_K1632007) to characterize the function of this part, by inserting fim switch [default ON](wild-type)(BBa_K1632004) upstream of a GFP coding sequence.

In the fimB dependent fim switch state assay, we transformed fim switch[default ON](wild-type)_rbs_gfp and PBAD/araC_fimB(BBa_K1632012)in the E.coli DH5alpha strain. We measured the fluorescence intensity of the cells induced by arabinose.

Similary,in the fimE dependent fim switch state assay, we transformed fim switch[default ON](wild-type)_rbs_gfp and PBAD/araC_fimE(wild-type)(BBa_K1632013) in the E.coli DH5alfpha strain. We measured the fluorescence intensity of the cells induced by arabinose.

For more information, see [http://2015.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2015 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1052