Difference between revisions of "Part:BBa K1603002:Design"

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Primers for isolation of the gene from genomic DNA with BioBrick Prefix in the FW primer and Suffix in the RV primer:
 
Primers for isolation of the gene from genomic DNA with BioBrick Prefix in the FW primer and Suffix in the RV primer:
 
FW:GCTTCTAGAGGTTTAAAGATTACGGATATTTAACTTACTTAGAATAATG
 
FW:GCTTCTAGAGGTTTAAAGATTACGGATATTTAACTTACTTAGAATAATG
 +
 
RV:AGCCTGCAGCGGCCGCTACTAGTATTTTAGTTTATGTATGTGTTTTTTGTAGTTATAGAT
 
RV:AGCCTGCAGCGGCCGCTACTAGTATTTTAGTTTATGTATGTGTTTTTTGTAGTTATAGAT
 +
 +
 +
The primers were designed to remove the EcoRI site from the insert when constructing the biobrick to reduce the amount of base pairs in the primers. The primers also add 3 extra protective basepairs to the PstI site in the insert.
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The construction of the final biobrick was initiated by cutting [https://parts.igem.org/Part:BBa_J04450 BBa_J04450] with XbaI, PstI, KnpI and FastAP and the insert with XbaI and PstI.
  
 
===Source===
 
===Source===
  
−
Genomic sequence from Saccharomyces cerevisiae CEN.PK2
+
Genomic sequence from ''Saccharomyces cerevisiae'' CEN.PK2
  
 
===References===
 
===References===

Latest revision as of 14:32, 18 September 2015


Promoter pTPI1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene from genomic DNA with BioBrick Prefix in the FW primer and Suffix in the RV primer: FW:GCTTCTAGAGGTTTAAAGATTACGGATATTTAACTTACTTAGAATAATG

RV:AGCCTGCAGCGGCCGCTACTAGTATTTTAGTTTATGTATGTGTTTTTTGTAGTTATAGAT


The primers were designed to remove the EcoRI site from the insert when constructing the biobrick to reduce the amount of base pairs in the primers. The primers also add 3 extra protective basepairs to the PstI site in the insert.


The construction of the final biobrick was initiated by cutting BBa_J04450 with XbaI, PstI, KnpI and FastAP and the insert with XbaI and PstI.

Source

Genomic sequence from Saccharomyces cerevisiae CEN.PK2

References