Difference between revisions of "Part:BBa K1603004"

 
 
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<partinfo>BBa_K1603004 short</partinfo>
 
<partinfo>BBa_K1603004 short</partinfo>
  
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Single strand binding protein from Deinococcus Radiodurans. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
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Single strand binding protein from ''Deinococcus radiodurans''. Codon optimized for ''Saccharomyces cerevisiae'' and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
  
  

Latest revision as of 14:30, 18 September 2015

SSB - optimized for yeast

Single strand binding protein from Deinococcus radiodurans. Codon optimized for Saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 248
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 111
    Illegal XhoI site found at 875
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 248
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 248
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 482