Difference between revisions of "Part:BBa K1859024"
Line 26: Line 26:
  
 
<p>
 
<p>
−
Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004].
+
Linkers designed to adjust folding of protein are in
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859003" > [BBa_K1859003] </a>
 +
and  
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859004" > [BBa_K1859004] </a>
 +
.
 
RNA sequences of these linkers bind complementary with each other in circular mRNA.  
 
RNA sequences of these linkers bind complementary with each other in circular mRNA.  
 
</p>
 
</p>
  
 
<p>
 
<p>
−
We found that circular efficiency of [BBa_1859026] was the third, when we compared with amount of circular mRNA made by these generators (BBa_1332011), (BBa_1859024), (BBa_18559025), (BBa_1859026).
+
We found that circular efficiency of [BBa_1859024] was the third, when we compared with amount of circular mRNA made by these generators  
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1332011" > [BBa_1332011] </a>
 +
, [BBa_1859024],  
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859025" > [BBa_K1859025] </a>
 +
,  
 +
<a href= "https://parts.igem.org/wiki/index.php?title=Part:BBa_K1859026" > [BBa_K1859026] </a>
 +
.
 
</p>
 
</p>
 
</html>
 
</html>

Revision as of 14:06, 18 September 2015

mRNA circularize efficiently generator (outside complementarity)

We designed this generator [K1859024] to improve circular efficiency of mRNA including His-RFP sequence.

The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .

Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004] . RNA sequences of these linkers bind complementary with each other in circular mRNA.

We found that circular efficiency of [BBa_1859024] was the third, when we compared with amount of circular mRNA made by these generators [BBa_1332011] , [BBa_1859024], [BBa_K1859025] , [BBa_K1859026] .