Difference between revisions of "Part:BBa K1789013"

Revision as of 14:06, 18 September 2015

SCAF1+Ter

This is a combination of scaffold and termination.

Usage and Biology

Usage: This part is scaf1 which is associated with a terminator. Scaf1 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.

Biology: Scaf1 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf1 with a terminator after it.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 145
Illegal AgeI site found at 175
Illegal AgeI site found at 205
Illegal AgeI site found at 235
Illegal AgeI site found at 265
Illegal AgeI site found at 295
Illegal AgeI site found at 325
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

CHIp

Enzyme digestion test /

@@ Line 4: / Line 4: @@
 This is a combination of scaffold and termination.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Usage:
+This part is scaf1 which is associated with a terminator. Scaf1 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.
+Biology:
+Scaf1 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf1 with a terminator after it.
 <!-- -->
@@ Line 12: / Line 18: @@
-<!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K1789013 parameters</partinfo>
 <!-- -->
+CHIp
-Usage:
+Enzyme digestion test
-This part is scaf1 which is associated with a terminator. Scaf1 is used to bind TALE1 and TALE3 protein to make them fixed on the DNA. We create this part to make it more convenient every time we are required to add a scaffold.
+/
-Biology:
-Scaf1 is a DNA scaffold which can bind TALE protein specifically to achieve the fixing of protein or enzyme. This part is scaf1 with a terminator after it.