Difference between revisions of "Part:BBa K1859024"
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His-RFPのmRNAを環状化させる効率を上げるために、私たちはこのジェネレーター[K1859024]をデザインした。
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We designed this generator [K1859024] to improve circular efficiency of mRNA including His-RFP sequence.
 
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BBa_K1859003とBBa_K1859004には、タンパク質のフォールディングを整えるために設計したリンカーが入っている。
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Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004].
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これらのリンカーのRNA配列は環状RNAの内部で相補的に結合する。
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RNA sequences of these linkers bind complementary with each other in circular mRNA.
 
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We found that circular efficiency of [BBa_1859026] was the best, when we compared with amount of circular mRNA made by these generators (BBa_1332011), (BBa_1859024), (BBa_18559025), (BBa_1859026).
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We found that circular efficiency of [BBa_1859026] was the third, when we compared with amount of circular mRNA made by these generators (BBa_1332011), (BBa_1859024), (BBa_18559025), (BBa_1859026).
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[BBa_1859024]は3番目に環状化の効率が良かった。
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Revision as of 13:17, 18 September 2015

mRNA circularize efficiently generator (outside complementarity)

We designed this generator [K1859024] to improve circular efficiency of mRNA including His-RFP sequence.

The generator is comprised of Circular parts combined the linker( [BBa_K1859003] and [BBa_K1859004] ), His-RFP without stop codon [BBa_K1332002] , Lacl [BBa_R0010] , RBS [BBa_B0034] and DT [BBa_B0015] .

Linkers designed to adjust folding of protein are in [BBa_K1859003] and [BBa_K1859004]. RNA sequences of these linkers bind complementary with each other in circular mRNA.

We found that circular efficiency of [BBa_1859026] was the third, when we compared with amount of circular mRNA made by these generators (BBa_1332011), (BBa_1859024), (BBa_18559025), (BBa_1859026).