Difference between revisions of "Part:BBa K1638036"
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| − | This part consist of an intein from Saccharomyces cerevisiae VMA1 gene including a CBD-tag. This intein is a thiol-induced self-cleavable protein that enables the release of a C-terminal fused protein. The chitin-binding domain is an affinity tag that enables affinity purification on a chitin column. First the target protein fused to intein is loaded and washed on the chitin column. Using a thiol reagent, like dithiothreitol (DTT), an on-column cleavage is induced and the target protein is released [1]. | + | This part consist of an intein from <i>Saccharomyces cerevisiae</i> <i>VMA1</i> gene including a chitin-binding domain (CBD)-tag. This intein is a thiol-induced self-cleavable protein that enables the release of a C-terminal fused protein. The chitin-binding domain is an affinity tag that enables affinity purification on a chitin column. First the target protein fused to intein is loaded and washed on the chitin column. Using a thiol reagent, like dithiothreitol (DTT), an on-column cleavage is induced and the target protein is released [1]. |
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Latest revision as of 13:05, 18 September 2015
Sce VMA Intein with promoter
This part consist of an intein from Saccharomyces cerevisiae VMA1 gene including a chitin-binding domain (CBD)-tag. This intein is a thiol-induced self-cleavable protein that enables the release of a C-terminal fused protein. The chitin-binding domain is an affinity tag that enables affinity purification on a chitin column. First the target protein fused to intein is loaded and washed on the chitin column. Using a thiol reagent, like dithiothreitol (DTT), an on-column cleavage is induced and the target protein is released [1].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 897
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 563
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1440
- 1000COMPATIBLE WITH RFC[1000]
References
[1] IMPACT™ Kit. 2015. New England Biolabs; [accessed 2015 Sep 11]. https://www.neb.com/products/e6901-impact-kit