Difference between revisions of "Part:BBa K1638010"

Revision as of 12:22, 18 September 2015

T25 domain of cyaA from Bordetella pertussis with GFP reporter

T25 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This part allows detection of gene expression. Additionally, the presence of green fluorescence will verify correct folding of the protein fused to the T18-domain through the flexible aa linker. Correct folding of our T18-domain is obviously improtant for the bacterial two-hybrid system to work.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 843
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1516

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1638010 short</partinfo>
-T25 domain of the catalytic active domain of CyaA from Bordetella pertussis linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This is part is primarily used to verify correct folding of the protein fused to the T25-domain through the flexible aa linker. Correct protein folding of the fusion protein is important for the purpose of the Bacterial Two-Hybrid System.
+T25 domain of the catalytic active domain of CyaA from <i>Bordetella pertussis</i> linked together with green fluorescent protein (GFP) through a flexible 10 aa linker. This part allows detection of gene expression. Additionally, the presence of green fluorescence will verify correct folding of the protein fused to the T18-domain through the flexible aa linker. Correct folding of our T18-domain is obviously improtant for the bacterial two-hybrid system to work.
 <!-- Add more about the biology of this part here