Difference between revisions of "Part:BBa K1603002:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Addition of Prefix and Suffix by using primers with overhangs in a PCR.
+
Primers for isolation of the gene from genomic DNA with BioBrick Prefix in the FW primer and Suffix in the RV primer:
−
 
+
FW:GCTTCTAGAGGTTTAAAGATTACGGATATTTAACTTACTTAGAATAATG
−
 
+
RV:AGCCTGCAGCGGCCGCTACTAGTATTTTAGTTTATGTATGTGTTTTTTGTAGTTATAGAT
  
 
===Source===
 
===Source===

Revision as of 12:17, 18 September 2015


Promoter pTPI1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene from genomic DNA with BioBrick Prefix in the FW primer and Suffix in the RV primer: FW:GCTTCTAGAGGTTTAAAGATTACGGATATTTAACTTACTTAGAATAATG RV:AGCCTGCAGCGGCCGCTACTAGTATTTTAGTTTATGTATGTGTTTTTTGTAGTTATAGAT

Source

Genomic sequence from Saccharomyces cerevisiae CEN.PK2

References