Difference between revisions of "Part:BBa K1729001"

Revision as of 12:11, 18 September 2015

Yeast mitochondrial ribosomal protein MRPS12

This translational unit includes the Kozak and codon-optimized sequence for the Saccharomyces cerevisiae [http://www.yeastgenome.org/locus/S000005319/overview MRPS12] gene, which encodes a ribosomal protein of the small subunit of the mitochondrial ribosome. The coding sequence begins with a mitochondrial localization signal, which is presumably removed in the mitochondrion. The resultant protein is a homologue of the highly conserved uS12 ribosomal protein (Ban et al.).

The part sequence corresponds to nucleotides 694840-694361 on chromosome XIV of the S. cerevisiae S288C reference genome.

Ban, Nenad et al. “A New System for Naming Ribosomal Proteins.” Current opinion in structural biology 24 (2014): 165–169.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 442
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1729001 short</partinfo>
-This translational unit includes the Kozak and codon-optimized sequence for the ''Saccharomyces cerevisiae'' [http://www.yeastgenome.org/locus/S000005319/overview ''MRPS12''] gene, which encodes an essential ribosomal protein of the small subunit of the mitochondrial ribosome. The coding sequence begins with a mitochondrial localization signal, which is presumably removed in the mitochondrion. The resultant protein is a homologue of the highly conserved uS12 ribosomal protein (Ban ''et al''.).
+This translational unit includes the Kozak and codon-optimized sequence for the ''Saccharomyces cerevisiae'' [http://www.yeastgenome.org/locus/S000005319/overview ''MRPS12''] gene, which encodes a ribosomal protein of the small subunit of the mitochondrial ribosome. The coding sequence begins with a mitochondrial localization signal, which is presumably removed in the mitochondrion. The resultant protein is a homologue of the highly conserved uS12 ribosomal protein (Ban ''et al''.).
 The part sequence corresponds to nucleotides 694840-694361 on chromosome XIV of the ''S. cerevisiae'' S288C reference genome.