Difference between revisions of "Part:BBa K1813061"

Latest revision as of 12:10, 18 September 2015

ptac pelB AgCPR pelb CYP6G1

With ptac powering the transcription of BBa_K1813053 and BBa_K1813046, this part allows the co-localization of large amounts of simultaneously expressed CYP6G1 ( BBa_K1813004) and A. gambiae NADPH p450 reductase ( BBa_K1813010) to the bacterial periplasm.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 626
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 141
Illegal NgoMIV site found at 2448
Illegal NgoMIV site found at 3394
Illegal AgeI site found at 424
Illegal AgeI site found at 1606
Illegal AgeI site found at 1762
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2122
Illegal BsaI.rc site found at 3403

Background

This part is also used in BBa_K1813073, BBa_K1813067 and BBa_K1813069

Revision as of 11:43, 18 September 2015 (view source) Rc (Talk \| contribs) ← Older edit		Latest revision as of 12:10, 18 September 2015 (view source) Rc (Talk \| contribs)
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	<partinfo>BBa_K1813061 short</partinfo>		<partinfo>BBa_K1813061 short</partinfo>

−	With ptac powering the transcription of <html><b> <a href="https://parts.igem.org/Part:BBa_K1813053">BBa_K1813053</a></b></html> and <html><b> <a href="https://parts.igem.org/Part:~~BBa_K1813004~~">~~BBa_K1813004~~</a></b></html>, this part allows the co-localization of large amounts of simultaneously expressed CYP6G1 and <i>A. gambiae</i> NADPH p450 reductase to the bacterial periplasm.	+	With ptac powering the transcription of <html><b> <a href="https://parts.igem.org/Part:BBa_K1813053">BBa_K1813053</a></b></html> and <html><b> <a href="https://parts.igem.org/Part:BBa_K1813046">BBa_K1813046</a></b></html>, this part allows the co-localization of large amounts of simultaneously expressed CYP6G1 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813004">BBa_K1813004</a></b></html>) and <i>A. gambiae</i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) to the bacterial periplasm.