Difference between revisions of "Part:BBa K1813061"

 
 
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<partinfo>BBa_K1813061 short</partinfo>
 
<partinfo>BBa_K1813061 short</partinfo>
  
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ptac pelB AgCPR pelb CYP6G1
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With ptac powering the transcription of <html><b> <a href="https://parts.igem.org/Part:BBa_K1813053">BBa_K1813053</a></b></html> and <html><b> <a href="https://parts.igem.org/Part:BBa_K1813046">BBa_K1813046</a></b></html>, this part allows the co-localization of large amounts of simultaneously expressed CYP6G1 (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813004">BBa_K1813004</a></b></html>) and <i>A. gambiae</i> NADPH p450 reductase (<html><b> <a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</a></b></html>) to the bacterial periplasm.
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<partinfo>BBa_K1813061 parameters</partinfo>
 
<partinfo>BBa_K1813061 parameters</partinfo>
 
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<h2> Background </h2>
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This part is also used in <html><b> <a href="https://parts.igem.org/Part:BBa_K1813073">BBa_K1813073</a></b></html>, <html><b> <a href="https://parts.igem.org/Part:BBa_K1813067">BBa_K1813067</a></b></html> and <html><b> <a href="https://parts.igem.org/Part:BBa_K1813069">BBa_K1813069</a></b></html>

Latest revision as of 12:10, 18 September 2015

ptac pelB AgCPR pelb CYP6G1

With ptac powering the transcription of BBa_K1813053 and BBa_K1813046, this part allows the co-localization of large amounts of simultaneously expressed CYP6G1 ( BBa_K1813004) and A. gambiae NADPH p450 reductase ( BBa_K1813010) to the bacterial periplasm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 626
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 141
    Illegal NgoMIV site found at 2448
    Illegal NgoMIV site found at 3394
    Illegal AgeI site found at 424
    Illegal AgeI site found at 1606
    Illegal AgeI site found at 1762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2122
    Illegal BsaI.rc site found at 3403


Background

This part is also used in BBa_K1813073, BBa_K1813067 and BBa_K1813069