Difference between revisions of "Part:BBa K1642011:Design"
Line 8: Line 8:
 
Pdark
 
Pdark
  
−
HR
+
HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)
  
 
Pdark-HR
 
Pdark-HR

Revision as of 10:58, 18 September 2015

Expression of Halorhodopsin under the control of Pdrak


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 783
    Illegal NgoMIV site found at 942
    Illegal NgoMIV site found at 1263
    Illegal AgeI site found at 1134
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Pdark

HR(http://2015.igem.org/Team:SJTU-BioX-Shanghai/Transport_Module)

Pdark-HR

Source

Overlap PCR from basic parts Up(BBa_K1642005), Pdark(BBa_K1026009), RBS(BBa_K1642004), HR(BBa_K1642001), Terminator(BBa_B0012), PcpcB-Kana(BBa_K1642003), Down(BBa_K1642006).