Difference between revisions of "Part:BBa K1734001"
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The immediate-early 2 promoter from the multicapsid nucleopolyhedrosis virus Orgyia pseudotsugata (OpIE2) is a constitutive promoter. Usually is used in nonlytic gene expression systems [1], but can be used with the baculovirus expression vector system (BEVS) methodology as well. | The immediate-early 2 promoter from the multicapsid nucleopolyhedrosis virus Orgyia pseudotsugata (OpIE2) is a constitutive promoter. Usually is used in nonlytic gene expression systems [1], but can be used with the baculovirus expression vector system (BEVS) methodology as well. | ||
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| + | https://static.igem.org/mediawiki/2015/c/cf/Tec_Monterrey_Conf_Opie2.jpg | ||
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| + | This part has been confirmed by restriction analysis of the pSB1C3 vector | ||
Latest revision as of 10:45, 18 September 2015
OpIE2 promoter
The immediate-early 2 promoter from the multicapsid nucleopolyhedrosis virus Orgyia pseudotsugata (OpIE2) is a constitutive promoter. Usually is used in nonlytic gene expression systems [1], but can be used with the baculovirus expression vector system (BEVS) methodology as well.
This part has been confirmed by restriction analysis of the pSB1C3 vector
Reference:
[1]Kempf, J., Snook, L. A., Vonesch, J.-L., Dahms, T. E. S., Pattus, F., & Massotte, D. (2002). Expression of the human μ opioid receptor in a stable Sf9 cell line. Journal of Biotechnology, 95(2), 181-187. doi:http://dx.doi.org/10.1016/S0168-1656(02)00008-1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 290
- 1000COMPATIBLE WITH RFC[1000]