Difference between revisions of "Part:BBa K1732012"

Revision as of 09:18, 18 September 2015

pSB1C3-J23100-B0034-YFP-His-B0015

His tagged YFP and strong RBS. The J23100 strong constitutive promoter allows for high level expression of the fluorescent protein used to measure fluorescence.

A set of fluorescent proteins which includes BFP, GFP, eGFP, E0040, OFP, YFP, and RFP with a J23100 constitutive promoter and RFP with a J23115 constitutive promoter were characterized. The relative fluorescence as well as the signal-to-noise ratio of all the proteins in the MACH competent cell line were calculated. YFP was measured at (ex/em = 514nm/527nm).

Sequence alignment of original sequence to codon optimized sequence, indicating the differences in amino acids.

The graphs below show the data for the original set of fluorescent proteins prior to being codon optimized via the IDT codon optimization tool. The set consists of His tagged RFP, OFP, YFP, eGFP, and BFP, each with a strong RBS and WT lac promoter that allows for Lac induction of fluorescence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 77

@@ Line 14: / Line 14: @@
 [[File:FQuantitation.jpg]]
+----
+Sequence alignment of original sequence to codon optimized sequence, indicating the differences in amino acids.
+[[File:Alignment YFP.jpg]]
+----
+The graphs below show the data for the original set of fluorescent proteins prior to being codon optimized via the IDT codon optimization tool. The set consists of His tagged RFP, OFP, YFP, eGFP, and BFP, each with a strong RBS and WT lac promoter that allows for Lac induction of fluorescence.
+[[File:Fluorescent_proteins_characterization.jpg]]
+[[File:Fluorescent_proteins_Amounts.jpg]]
 <!-- Add more about the biology of this part here