Difference between revisions of "Part:BBa K1628007"
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| − | Promoter PamyA is a strong promoter in Bacillus amyloliquefaciens LL3. According to our promoter strength assay in (showed in Figure 1), PamyA is the second strongest promoter in our project. | + | Promoter PamyA is a strong promoter in Bacillus amyloliquefaciens LL3. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in (showed in Figure 1), PamyA is the second strongest promoter in our project. |
| − | [[File: | + | [[File:promoter4_NK.png]] |
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Latest revision as of 09:07, 18 September 2015
PamyA
Promoter PamyA is a strong promoter in Bacillus amyloliquefaciens LL3. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to our promoter strength assay in (showed in Figure 1), PamyA is the second strongest promoter in our project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 178
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]