Difference between revisions of "Part:BBa K1694034"
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<partinfo>BBa_K1694034 short</partinfo> | <partinfo>BBa_K1694034 short</partinfo> | ||
| − | + | <h1>'''Introduction:'''</h1> | |
| − | + | The most commonly constructing way of composite parts is to ligate the required parts together. We also provide this kind of parts. | |
| + | <br> | ||
| + | At the back of the Lpp-OmpA-scFv part, we ligated the weaker ribosome biding site (BBa_B0030), different fluorescent protein and terminator (BBa_J61048) to make it continuously and simultaneously express the fluorescence and the scFv. We used a weak ribosome binding site to ensure that scFv's production will not be affected. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 09:07, 18 September 2015
Pcons+B0034+Lpp-OmpA-N+scFv(Anti-EGFR)+B0030+GFP+J61048
Introduction:
The most commonly constructing way of composite parts is to ligate the required parts together. We also provide this kind of parts.
At the back of the Lpp-OmpA-scFv part, we ligated the weaker ribosome biding site (BBa_B0030), different fluorescent protein and terminator (BBa_J61048) to make it continuously and simultaneously express the fluorescence and the scFv. We used a weak ribosome binding site to ensure that scFv's production will not be affected.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 451
Illegal NgoMIV site found at 2001 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1917