Difference between revisions of "Part:BBa K1628003"
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| − | Promoter BJ27UP is an artificially synthesized promoter. According to | + | Promoter BJ27UP is an artificially synthesized promoter. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in ''B. amyloliquefaciens'' NK-1 (showed in Figure 1), the strength of BJ27UP is stronger than Pbca but is still too weak compared with other promoters. |
| − | [[File: | + | [[File:promoter4_NK.png]] |
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Latest revision as of 09:04, 18 September 2015
BJ27UP
Promoter BJ27UP is an artificially synthesized promoter. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in B. amyloliquefaciens NK-1 (showed in Figure 1), the strength of BJ27UP is stronger than Pbca but is still too weak compared with other promoters.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]