Difference between revisions of "Part:BBa K1628102"
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''pgsC'' and ''pgsA'' are two genes in ''pgsBCA'' operon. Protein PgsBCA is a membrane protein responsible for the synthesis of γ-PGA. Subunit PgsC is responsible for glutamic acid’s polymerization and subunit PgsA is responsible for the secretion of γ-PGA (showed in Figure 3). | ''pgsC'' and ''pgsA'' are two genes in ''pgsBCA'' operon. Protein PgsBCA is a membrane protein responsible for the synthesis of γ-PGA. Subunit PgsC is responsible for glutamic acid’s polymerization and subunit PgsA is responsible for the secretion of γ-PGA (showed in Figure 3). | ||
| − | [[File: | + | [[File:pgsBCA1_NK.png]] |
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| + | Funtion of part pgsB and pgsCA were validated through fermentation experiment. Wild type ''Bacillus amyloliquefaciens'' NK-1 strain could produce 3-4g/L γ-PGA after 48 hours of fermentation. In previous work in our laboratory, we also tested heterologous expression of γ-PGA in ''E. coli''. We constructed different expression vectors containing ''pgsBCA'' genes and transformed them separately into ''E.coli'' JM109. The average production of γ-PGA in ''E. coli'' JM109 is around 0.5g/L. | ||
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| + | [[File:pgsBCA2_NK.png]] | ||
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Latest revision as of 08:19, 18 September 2015
pgsCA
pgsC and pgsA are two genes in pgsBCA operon. Protein PgsBCA is a membrane protein responsible for the synthesis of γ-PGA. Subunit PgsC is responsible for glutamic acid’s polymerization and subunit PgsA is responsible for the secretion of γ-PGA (showed in Figure 3).
Funtion of part pgsB and pgsCA were validated through fermentation experiment. Wild type Bacillus amyloliquefaciens NK-1 strain could produce 3-4g/L γ-PGA after 48 hours of fermentation. In previous work in our laboratory, we also tested heterologous expression of γ-PGA in E. coli. We constructed different expression vectors containing pgsBCA genes and transformed them separately into E.coli JM109. The average production of γ-PGA in E. coli JM109 is around 0.5g/L.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]