Difference between revisions of "Part:BBa K1684005"

 
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Yellow chromoprotein optimized for E.coli K12 with the standar Terminator
 
Yellow chromoprotein optimized for E.coli K12 with the standar Terminator
  
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===Usage and Biology===
 
===Usage and Biology===
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Chromoproteins are a collection of proteins that express pigments of strong colors. The AmilGFP derivate from Acropora millepora, present a yellow pigment when it’s expressed. Also, the protein has a maximum absorption of 512 nm.
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This version with the standar terminator is codon optimized for the expression in E.coli K12.
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===Results===
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In Figure 1 samples composite parts are presented, the first BBa_K1684004, is composed of the chromoprotein sequence optimized for E. coli K12 spisPink (BBa_K1684000) and a terminator (BBa_B0010); the second part is composed of the chromoprotein optimized sequence amilGFP (BBa_K1684001) and the same terminator (BBa_B0010). This agarose gel shown on Line 3 and 4 the composite BioBrick BBa_K1684005, in the first case the result was not successful; In Line 4 however, the band is present with the respective size, 2890 bp, as a positive result. Finally on Line 5 is presented only the negative control.
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<img class="responsive-img" src="https://static.igem.org/mediawiki/2015/b/b9/GEL3BIOSINT2015.png
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1684005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1684005 SequenceAndFeatures</partinfo>
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===References===
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Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680.
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doi:10.1371/journal.pone.0002680
  
  

Latest revision as of 08:11, 18 September 2015

amilGFP + Terminator

Yellow chromoprotein optimized for E.coli K12 with the standar Terminator

Usage and Biology

Chromoproteins are a collection of proteins that express pigments of strong colors. The AmilGFP derivate from Acropora millepora, present a yellow pigment when it’s expressed. Also, the protein has a maximum absorption of 512 nm.

This version with the standar terminator is codon optimized for the expression in E.coli K12.

Results

In Figure 1 samples composite parts are presented, the first BBa_K1684004, is composed of the chromoprotein sequence optimized for E. coli K12 spisPink (BBa_K1684000) and a terminator (BBa_B0010); the second part is composed of the chromoprotein optimized sequence amilGFP (BBa_K1684001) and the same terminator (BBa_B0010). This agarose gel shown on Line 3 and 4 the composite BioBrick BBa_K1684005, in the first case the result was not successful; In Line 4 however, the band is present with the respective size, 2890 bp, as a positive result. Finally on Line 5 is presented only the negative control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680