Difference between revisions of "Part:BBa K1684001"

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This version is codon optimized for the expression in E.coli K12.  
 
This version is codon optimized for the expression in E.coli K12.  
  
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===Results===
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The Figure 1 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 4,5 and 6 are repeated samples of the piece amilGFP optimized for E. coli K12 (BBa_K1684001). For both BioBricks, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.
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<img class="responsive-img" src="https://static.igem.org/mediawiki/2015/9/97/GELBIOSINT2015.png" width="60%" style="position: center; top:0%;"/>
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<partinfo>BBa_K1684001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1684001 SequenceAndFeatures</partinfo>
  
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===References===
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Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680.
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doi:10.1371/journal.pone.0002680
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 08:06, 18 September 2015

amilGFPK12

Yellow chromoprotein optimized for E.coli k12

Usage and Biology

Chromoproteins are a collection of proteins that express pigments of strong colors. The AmilGFP derivate from Acropora millepora, present a yellow pigment when it’s expressed. Also, the protein has a maximum absorption of 512 nm.

This version is codon optimized for the expression in E.coli K12.

Results

The Figure 1 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 4,5 and 6 are repeated samples of the piece amilGFP optimized for E. coli K12 (BBa_K1684001). For both BioBricks, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 612
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680