Difference between revisions of "Part:BBa K1684000"
 
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Pink chromoprotein optimized for E.coli k12  
 
Pink chromoprotein optimized for E.coli k12  
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===Usage and Biology===
 
===Usage and Biology===
  
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Chromoproteins are a collection of proteins that express pigments of strong colors.  
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Chromoproteins are a collection of proteins that express pigments of strong colors. This protein, expressed in ''Stylophora pistillata'', was evolving independently from the rest of coral chromoproteins.
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The protein has a maximum absorption of 560 nm. This characteristic confers a blue shift by at least 14 nanometers in comparison to other known coral chromoproteins, inducing the production of pink color rather than purple appearance.
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This version is codon optimized for the expression in E.coli K12.
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===Results===
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The Figure 1 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 1, 2 and 3 are located the triplicate samples of spisPink piece, optimized for E. coli K12 (BBa_K1684000), with a size of 2789 bp. For the BioBrick, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.
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<img class="responsive-img" src="https://static.igem.org/mediawiki/2015/9/97/GELBIOSINT2015.png" width="60%" style="position: center; top:0%;"/>
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<partinfo>BBa_K1684000 parameters</partinfo>
 
<partinfo>BBa_K1684000 parameters</partinfo>
 
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===References===
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Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680.
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doi:10.1371/journal.pone.0002680

Latest revision as of 08:04, 18 September 2015

spisPinkK12

Pink chromoprotein optimized for E.coli k12

Usage and Biology

Chromoproteins are a collection of proteins that express pigments of strong colors. This protein, expressed in Stylophora pistillata, was evolving independently from the rest of coral chromoproteins.

The protein has a maximum absorption of 560 nm. This characteristic confers a blue shift by at least 14 nanometers in comparison to other known coral chromoproteins, inducing the production of pink color rather than purple appearance.

This version is codon optimized for the expression in E.coli K12.

Results

The Figure 1 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 1, 2 and 3 are located the triplicate samples of spisPink piece, optimized for E. coli K12 (BBa_K1684000), with a size of 2789 bp. For the BioBrick, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680