Difference between revisions of "IGEM:Bordeaux/2015/stationary phase curdlan synthase"

(Created page with "https://static.igem.org/mediawiki/2015/thumb/1/16/Bordeaux_Team_promoter_characterizationV4.png/800px-Bordeaux_Team_promoter_characterizationV4.png '''Growth dependent banana odor pro...")
 
 
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'''Growth dependent banana odor production'''<br>
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'''Curdlan synthase under the expression of stationary phase promoter'''<br>
  
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To demonstrate growth phase dependent banana odor production, we compared the behavior of constitutive and stationary phase dependent banana odor generators (A and B, respectively). We measured isoamyl acetate concentrations of cultures of the constitutive and stationary phase banana odor generators at different culture cell densities (OD600nm) (C). As expected, the stationary phase banana odor generator produced very little isoamyl acetate at low cell densities but its isoamyl acetate production increased with cell density.  By comparison, the constitutive banana odor generator produced more isoamyl acetate at lower cell densities than the stationary phase banana odor generator. To visually aid comparison of the two odor generators, an empirical fit to the data for each device is shown.]]
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To demonstrate growth phase dependent activation of our Curdlan synthase gene, we compared the amount of Curdlan produced by ''E.coli'' while at the same time looking at OD to measure growth. The Curdlan concentration was calculated with absorption measures (excitation wavelength at 398nm and emission wavelength= 502nm) after Curdlan purification from cells. We used aniline blue dye since it is specific of beta glucans.

Latest revision as of 07:58, 18 September 2015

800px-Bordeaux_Team_promoter_characterizationV4.png

Curdlan synthase under the expression of stationary phase promoter

To demonstrate growth phase dependent activation of our Curdlan synthase gene, we compared the amount of Curdlan produced by E.coli while at the same time looking at OD to measure growth. The Curdlan concentration was calculated with absorption measures (excitation wavelength at 398nm and emission wavelength= 502nm) after Curdlan purification from cells. We used aniline blue dye since it is specific of beta glucans.