Difference between revisions of "Part:BBa K1813018"

Latest revision as of 07:37, 18 September 2015

AgambNADPH Expression Cassette

A construct used to achieve the overexpression of the A. gambiae NADPH p450 reductase (BBa_K1813010) in a bacterial host.

Structure: ptac:: AgambNADPH:: terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 560
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 358
Illegal AgeI site found at 1540
Illegal AgeI site found at 1696
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2056

Background

Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of the A. gambiae NADPH p450 reductase by any bacterium harboring this construct. This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813028.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1813018 short</partinfo>
-ptac AgambNADPH term
+A construct used to achieve the overexpression of the <i> A. gambiae</i> NADPH p450 reductase (<html><b><a href="https://parts.igem.org/Part:BBa_K1813010">BBa_K1813010</b></a></html>)  in a bacterial host.
+Structure: ptac:: AgambNADPH:: terminator.
@@ Line 18: / Line 19: @@
 <partinfo>BBa_K1813018 parameters</partinfo>
 <!-- -->
+<h2> Background </h2>
+Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of the <i> A. gambiae</i> NADPH p450 reductase by any bacterium harboring this construct.
+This construct was assembled by Michael VanInsberghe, and is codon optimized for <i> E. coli </i>. <br>
+It is used in <html><b><a href="https://parts.igem.org/Part:BBa_K1813028">BBa_K1813028</b></a></html>.