Difference between revisions of "Part:BBa K1819000"
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<center><img src=https://static.igem.org/mediawiki/2015/5/52/Brasil-USP_NdeIlinkerGFP.png width= "300"> </img src><br>
 
<center><img src=https://static.igem.org/mediawiki/2015/5/52/Brasil-USP_NdeIlinkerGFP.png width= "300"> </img src><br>
 
<p>Figure 1 - Schematic representation of BBa_K1819000 insert</p></center>
 
<p>Figure 1 - Schematic representation of BBa_K1819000 insert</p></center>
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<h3>Characterization</h3>
 
<h3>Characterization</h3>
 
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Revision as of 07:30, 18 September 2015

Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site.

It allows to characterize protein expression and secretion and also may serve to other IGEM teams that would like to express a chimera C-terminally attached to GFP, which exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.

We also added GGGS amino acid twice as a spacer to avoid interaction between two consecutive proteins, allowing proper folding. This strategy was already applied to BBa_K1489002 part.


Figure 1 - Schematic representation of BBa_K1819000 insert


Characterization


Figure 2 - Gel restriction analysis


This part was characterized using two constitutive promoters from Anderson library (BBa_J23106 and BBa_J23117). See BBa_K1819006 and BBa_K1819007 pages for more information.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 674