Difference between revisions of "Part:BBa K1819000"

Revision as of 07:09, 18 September 2015

Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site.

It allows to characterize protein expression and secretion and also may serve to other IGEM teams that would like to express a chimera C-terminally attached to GFP, which exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.

We also added GGGS amino acid twice as a spacer to avoid interaction between two consecutive proteins, allowing proper folding. This strategy was already applied to BBa_K1489002 part.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 674

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-Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site. <br>It allows to characterize protein expression and secretion and also may serve to other IGEM teams that would like to express a chimera C-terminally attached to GFP, which exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
+Designed by Brasil-USP team, BBa_K1819000 is a GFP coding sequence with an N-terminal linker, which is 5’-flanked by NdeI restriction site. <br><br>It allows to characterize protein expression and secretion and also may serve to other IGEM teams that would like to express a chimera C-terminally attached to GFP, which exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
-<br>
+<br><br>
 We also added GGGS amino acid twice as a spacer to avoid interaction between two consecutive proteins, allowing proper folding. This strategy was already applied to BBa_K1489002 part.
 </p>