Difference between revisions of "Part:BBa K1813012"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1813012 short</partinfo> | <partinfo>BBa_K1813012 short</partinfo> | ||
− | A construct used to achieve the overexpression of CYP6G1 | + | A construct used to achieve the overexpression of CYP6G1(<html><b><a href="https://parts.igem.org/Part:BBa_K1813004">BBa_K181304</b></a></html>) <br> |
Structure: ptac:: CYP6G1:: terminator. | Structure: ptac:: CYP6G1:: terminator. | ||
Revision as of 06:33, 18 September 2015
CYP6G1 Expression Cassette
A construct used to achieve the overexpression of CYP6G1(BBa_K181304)
Structure: ptac:: CYP6G1:: terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1021
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1030
Background
Powered by the constitutive bacterial promoter ptac, this construct allows the indiscriminate production of CYP6G1 by a bacterium harbouring this construct.
This construct was assembled by Michael VanInsberghe, and is codon optimized for E. coli .
It is used in BBa_K1813025 and BBa_K1813033.