Difference between revisions of "Part:BBa K1804002:Design"

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===References===
 
===References===
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The nirB promoter of Escherichia coli: location of nucleotide sequences essential for regulation by oxygen, the FNR protein and nitrite. Molecular Microbioiogy (1988) 2(4), 527-530

Revision as of 05:42, 18 September 2015


pNirB-Invasin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

The invasin gene from Yersinia is derived from https://parts.igem.org/Part:BBa_K299812 and the pNirB promoter is derived from Jayaraman et al., (1988).

References

The nirB promoter of Escherichia coli: location of nucleotide sequences essential for regulation by oxygen, the FNR protein and nitrite. Molecular Microbioiogy (1988) 2(4), 527-530