Difference between revisions of "Part:BBa K1813031"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1813031 short</partinfo>
 
<partinfo>BBa_K1813031 short</partinfo>
Line 12: Line 11:
 
<partinfo>BBa_K1813031 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1813031 SequenceAndFeatures</partinfo>
  
−
 
−
<!-- Uncomment this to enable Functional Parameter display
 
−
===Functional Parameters===
 
 
<partinfo>BBa_K1813031 parameters</partinfo>
 
<partinfo>BBa_K1813031 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
<h2>Background of <i>LacI Reversed Ptac NicX Term</i></h2>
 +
 +
<h4>Part Description:</h4>
 +
<p>
 +
</p>
 +
 +
 +
<h4>Part Origin</h4>
 +
<p>where part originated and <i>Genus species</i> more words if needed</p>
 +
 +
<h4>Part Function</h4>
 +
<p>part functionstuff </p>
 +
 +
<h4>Design and Acquisition</h4>
 +
<p>
 +
After synthesizing a codon-optimized NicX, we used standard assembly to created a composite part composed of NicX driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicX expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicX. All nicX constructs are contained within standard pSB1C3 vectors.</p>
 +
 +
<h4>Experience</h4>
 +
<p><p>

Revision as of 05:38, 18 September 2015

nicX Expression Cassette with LacI Reversed

LacIR nicX

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 105
    Illegal BglII site found at 1746
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1895
    Illegal AgeI site found at 2123
    Illegal AgeI site found at 2321
  • 1000
    COMPATIBLE WITH RFC[1000]

Background of LacI Reversed Ptac NicX Term

Part Description:


Part Origin

where part originated and Genus species more words if needed

Part Function

part functionstuff

Design and Acquisition

After synthesizing a codon-optimized NicX, we used standard assembly to created a composite part composed of NicX driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicX expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicX. All nicX constructs are contained within standard pSB1C3 vectors.

Experience

<p>