Difference between revisions of "Part:BBa K1833008"
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<partinfo>BBa_K1833008 short</partinfo> | <partinfo>BBa_K1833008 short</partinfo> | ||
− | This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 9, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on this part, visit 2015.igem.org/Team:Pitt/Protease/Project. | + | This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 9, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on the use of this part, visit <html><a href="http://2015.igem.org/Team:Pitt/Protease/Project">the 2015 Pitt iGEM page.</a></html> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 05:29, 18 September 2015
pT7-MMP9 sensitive repressor
This part produces a synthetic repressor in response to T7 RNA Polymerase. This synthetic repressor consists of a fusion of two DNA binding domains connected with a linker that is sensitive to matrix metalloprotease 9, and it represses the promoter described by parts BBa_K1833001 and BBa_K1833003. For more information on the use of this part, visit the 2015 Pitt iGEM page.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 64
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 297
Illegal NgoMIV site found at 696 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 194
Illegal SapI.rc site found at 53