Difference between revisions of "Part:BBa K1813016"

After synthesizing a codon-optimized NicE, we used standard assembly to created a composite part composed of NicE driven by the Ptac promoter (BBa_K1813037) and flanked by a double terminator (BBa_B0014). The tac promoter contains a lac operator sequence that can be bound by LacI, the lac repressor protein, allowing inducible expression by Isopropyl β-D-1-thiogalactopyranoside (IPTG). Our NicE expression cassette was assembled behind a lacI cassette (BBa_K1813019) to give us the ability to control the expression of NicE. All nicE constructs are contained within standard pSB1C3 vectors.</p>