Difference between revisions of "Part:BBa K1628007"

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<partinfo>BBa_K1628007 short</partinfo>
 
<partinfo>BBa_K1628007 short</partinfo>
  
Promoter PamyA is a strong promoter in Bacillus amyloliquefaciens LL3. According to our promoter strength assay in (showed in Figure 1), PamyA is the second strongest promoter in our project.
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Promoter PamyA is a strong promoter in Bacillus amyloliquefaciens LL3. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter genes. According to our promoter strength assay in (showed in Figure 1), PamyA is the second strongest promoter in our project.
  
[[File:promoter2_NK.png]]
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[[File:promoter3_NK.png]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:30, 18 September 2015

PamyA

Promoter PamyA is a strong promoter in Bacillus amyloliquefaciens LL3. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter genes. According to our promoter strength assay in (showed in Figure 1), PamyA is the second strongest promoter in our project.

Promoter3 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 178
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]