Difference between revisions of "Part:BBa K1628001"

Revision as of 03:44, 18 September 2015

Pbca

Promoter Pbca is the original promoter of pgsBCA operon. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter genes. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1628001 short</partinfo>
-Promoter Pbca is the original promoter of ''pgsBCA'' operon. We have validate this part through promoter strength assay using  bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter genes. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak.
+Promoter Pbca is the original promoter of ''pgsBCA'' operon. We have validate this part through promoter strength assay using ''bgaB'' (encoding β-galatosidase downstream of the multiple cloning sites) as reporter genes. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak.
 [[File:promoter3_NK.png]]