Difference between revisions of "Part:BBa K1628001"
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| − | Promoter Pbca is the original promoter of ''pgsBCA'' operon. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter genes. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak. | + | Promoter Pbca is the original promoter of ''pgsBCA'' operon. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter genes. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak. |
| − | [[File: | + | [[File:promoter3_NK.png]] |
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Revision as of 03:36, 18 September 2015
Pbca
Promoter Pbca is the original promoter of pgsBCA operon. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter genes. According to the result of promoter strength assay (showed in Figure 1), the strength of Pbca is very weak.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]