Difference between revisions of "Part:BBa K1632022"

Revision as of 02:13, 18 September 2015

J23100_rbs_lasR_TT_Plux_rbs_CmRssrA

At the first stage of wet experiment, we used the cells which have the plasmid which is Pcon_lasR_TT_Plux_rbs_CmR to characterize the function of rbs_CmR(BBa_K395160 by iGEM10_Tokyo_Tech). But the cells showed active growth even in the absence of AHL (Fig. 1.The cells growth with Cm)

Fig. 1. The cells growth with Cm

From the results of this experiment, initially designed circuits showed leaky expression of CmR. So we inserted an ssrA degradation tag to the C-terminal of CmR.

Fig. 2. The cells growth with Cm

In the experiment using the Pcon_lasR_TT_Plux_cmRssrA (BBa_K1632022) and Pcon_rhlR_TT_Plux_cmRssrA (BBa_K1632023), we could not observe cell growth for cells that owned the ssrA-taged plasmid, in the absence of AHL
From our experiment, CmRssrA is confirmed work better than CmR without ssrA tag for our project.

For more information, see [http://2015.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2015 wiki].

Sequence and Features