Difference between revisions of "Part:BBa K1769002"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | <p>This sensor system consists of a negative feedback and positive feedback loop. The positive feedback loop is initiated when the inducible promoter, J61051, is induced by salicylic acid and produced protein LuxR. LuxR then forms a complex with AHL, a protein produced by LuxI. This complex upregulates the production of LuxI, Aiia, and GFP. However, once Aiia is matured, it will degrade AHL much faster then LuxI can produce and downregulate the production of GFP LuxI and Aiia itself. The production of Aiia will stop which allows the expression of Luxi to produce of AHL faster than Aiia can degrade.Moreover, AHL is a quorum sensing molecule which allows cells to synchronize thier production of GFP reporter.<p> | |
| − | <p>This sensor system consists of a negative feedback and positive feedback loop. The positive feedback loop is initiated when the inducible promoter, J61051, is induced by salicylic acid and produced protein LuxR. LuxR then forms a complex with AHL, a protein produced by LuxI. This complex upregulates the production of LuxI, Aiia, and GFP. However, once Aiia is matured, it will degrade AHL much faster then LuxI can produce and downregulate the production of GFP LuxI and Aiia itself. The production of Aiia will stop which allows the expression of Luxi to produce of AHL faster than Aiia can degrade.Moreover, AHL is a quorum sensing molecule which allows cells to synchronize thier production of GFP reporter.<p | + | |
==Result== | ==Result== | ||
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Revision as of 02:07, 18 September 2015
Salicylic acid sensing Oscillator
Inducible promoter,J61051,act as salicylic acid sensor, when the promoter is activated, it will produce luxI which in turn triggers the oscillator. GFP is the reporter of this circuit.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 786
Illegal BglII site found at 2938 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 3249 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2218
Illegal BsaI.rc site found at 3087
Illegal BsaI.rc site found at 4107
Illegal BsaI.rc site found at 4837