Difference between revisions of "Part:BBa K1694004"
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<partinfo>BBa_K1694004 short</partinfo>
 
<partinfo>BBa_K1694004 short</partinfo>
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<h1>'''Introduction:''' Single Chain Variable Fragment of Cetuximab</h1>
 
<h1>'''Introduction:''' Single Chain Variable Fragment of Cetuximab</h1>
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'''Features of scFv:'''
 
'''Features of scFv:'''
 
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'''1.specificity:'''the property of specificity of antibody to antigen has being maintained
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'''1.specificity:'''Though remove of the constant regions and add a linker, scFv still maintain the specificity of the original immunoglobulin.
 
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2.can be simply produced or displayed by bacteria such as E. coli
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'''2.Convenient：'''ScFv is smaller than the entire antibody, so that the loading of production to E.coli could become lower.
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<p style="font-size:120%">'''Cetuximab'''</p>
 
<p style="font-size:120%">'''Cetuximab'''</p>
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We selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab
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(International Nonproprietary Name (INN) : epidermal growth factor receptor (EGFR) inhibitor) is a chimeric (mouse/human) monoclonal antibody and it specific binds to target antigen epidermal growth factor receptor (EGFR).With high affinity it can prevent ligand binding and activation
 
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Epidermal growth factor receptor (EGFR) is a transmembrane tyrosine kinase receptor that regulate the cell division and cell apoptosis.  
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<p style="font-size:120%">'''Epidermal growth factor receptor (EGFR)'''</p>
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EGFR is a transmembrane tyrosine kinase receptor that regulate the cell division and cell apoptosis.  
 
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EGFR is characterized by an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic domain containing the tyrosine kinase region followed by a carboxyl-terminal tail with tyrosine autophosphorylation sites.
 
EGFR is characterized by an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic domain containing the tyrosine kinase region followed by a carboxyl-terminal tail with tyrosine autophosphorylation sites.
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EGFR is overexpressed on the cell surfaces of various solid tumors.
 
Mutations in the gene encoding EGFR that lead to overexpression of this protein will lead to cells proliferate uncontrollably.
 
Mutations in the gene encoding EGFR that lead to overexpression of this protein will lead to cells proliferate uncontrollably.
  
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<h1>'''Mechanism:'''
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:05, 18 September 2015

Single-chain variable fragment (Anti-EGFR)


Introduction: Single Chain Variable Fragment of Cetuximab

we selected the single chain variable fragments (scFv) of monoclonal antibodies Cetuximab and named it Anti-EGFR

scFv (Single Chain Variable Fragment)

scFv(single-chain variable fragment) is a fusion protein that consist of variable region of heavy chain(VH) and light chain(VL) of antibody, and connected by a short linker. Here we use the amino acid sequence of GGSSRSSSSGGGGSGGGG as a linker to connect VH and VL.

Features of scFv:
1.specificity:Though remove of the constant regions and add a linker, scFv still maintain the specificity of the original immunoglobulin.
2.Convenient：ScFv is smaller than the entire antibody, so that the loading of production to E.coli could become lower.

Cetuximab

(International Nonproprietary Name (INN) : epidermal growth factor receptor (EGFR) inhibitor) is a chimeric (mouse/human) monoclonal antibody and it specific binds to target antigen epidermal growth factor receptor (EGFR).With high affinity it can prevent ligand binding and activation

Epidermal growth factor receptor (EGFR)

EGFR is a transmembrane tyrosine kinase receptor that regulate the cell division and cell apoptosis.


EGFR is characterized by an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic domain containing the tyrosine kinase region followed by a carboxyl-terminal tail with tyrosine autophosphorylation sites.
EGFR is overexpressed on the cell surfaces of various solid tumors. Mutations in the gene encoding EGFR that lead to overexpression of this protein will lead to cells proliferate uncontrollably.

Mechanism: Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]