Difference between revisions of "Part:BBa K1795000:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K1795000 short</partinfo>
 
<partinfo>BBa_K1795000 short</partinfo>
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===Source===
 
===Source===
  
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The basic template for the sgRNA sequence was taken from the supplementary information of Qi LS, Larson MH, Gilbert LA, et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 2013;152(5):1173-1183. doi:10.1016/j.cell.2013.02.022.
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This sequence codes for dCas9 and is codon optimized for E. Coli. It will need a Promoter, RBS, and double terminator in order to function. Thanks to team iGEM13_SJTU-BioX-Shanghai  for original, non-codon optimized sequence.  
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===References===
 
===References===

Revision as of 01:32, 18 September 2015

E. Coli Codon Optimized dCAS9, solo


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 790
    Illegal PstI site found at 2416
    Illegal PstI site found at 3658
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 790
    Illegal PstI site found at 2416
    Illegal PstI site found at 3658
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 990
    Illegal BglII site found at 1046
    Illegal BglII site found at 1188
    Illegal BglII site found at 1736
    Illegal BglII site found at 3576
    Illegal BglII site found at 3815
    Illegal BamHI site found at 3378
    Illegal BamHI site found at 3739
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 790
    Illegal PstI site found at 2416
    Illegal PstI site found at 3658
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 790
    Illegal PstI site found at 2416
    Illegal PstI site found at 3658
    Illegal NgoMIV site found at 3960
    Illegal AgeI site found at 3304
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ensure the promoter targeted by this part is not used within this part.


Source

This sequence codes for dCas9 and is codon optimized for E. Coli. It will need a Promoter, RBS, and double terminator in order to function. Thanks to team iGEM13_SJTU-BioX-Shanghai for original, non-codon optimized sequence.

References