Difference between revisions of "Part:BBa K1766002:Design"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1766002 short</partinfo>
 
<partinfo>BBa_K1766002 short</partinfo>
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===Design Notes===
 
===Design Notes===
stop codon has to be removed
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This is a second version of a design that was faulty. The design was modified using site directed mutagenesis. The part contains residues from the faulty design, however, they should not interfere with the new design. For more detail about the making of this part, please visit the [http://2015.igem.org/Team:Stockholm/Results 2015 iGEM Stockholm wiki page] or contact [mailto:l.r.osterberg@gmail.com Linnea Österberg].
 
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===Source===
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e. coli
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===References===
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Latest revision as of 23:49, 17 September 2015

MicF regulated GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 861


Design Notes

This is a second version of a design that was faulty. The design was modified using site directed mutagenesis. The part contains residues from the faulty design, however, they should not interfere with the new design. For more detail about the making of this part, please visit the [http://2015.igem.org/Team:Stockholm/Results 2015 iGEM Stockholm wiki page] or contact Linnea Österberg.