Difference between revisions of "Part:BBa K1758353"

 
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<partinfo>BBa_K1758353 short</partinfo>
 
<partinfo>BBa_K1758353 short</partinfo>
  
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nickel repressor under teh control of a constitutive promoter with the induceble promoter a 5´untranslated region infront of the sfGFP which is used for detection.
  
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===Usage and Biology===
 
===Usage and Biology===
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<p> Our Nickel biosensor consists of parts of the rcn-operon from <i> E. coli </i> which codes for a nickel- and cobalt-efflux system. This system is highly sensitive to nickel. In absence of nickel or cobalt RcnR binds to the operator  and inhibits the nickel responsive promoter. With Ni(II)-ions present  the repression of the promoter RcnA will be reversed, because the repressor RcnR binds nickel-ions and cannot attach to the DNA. For our biosensor we construct the part (<a href="https://parts.igem.org/Part:BBa_K1758353" target="_blank"> BBa_K1758353 </a>by using the basic construction showed in <Our biosensors >. For this part we used the repressor RcnR under control of a constitutive promoter (<a href="https://parts.igem.org/Part:BBa_K1758350" target="_blank"> BBa_K1758350 </a>) and the nickel specific promoter RcnA with a 5’UTR  in front of sfGFP (<a href="https://parts.igem.org/Part:BBa_K1758352" target="_blank"> BBa_K1758352 </a>) as reporter protein. </p></html>
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1758353 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1758353 SequenceAndFeatures</partinfo>

Revision as of 23:31, 17 September 2015

Nickel repressor under control of constitutive promoter and strong RBS and Nickel responsive promote

nickel repressor under teh control of a constitutive promoter with the induceble promoter a 5´untranslated region infront of the sfGFP which is used for detection.

Usage and Biology

Our Nickel biosensor consists of parts of the rcn-operon from E. coli which codes for a nickel- and cobalt-efflux system. This system is highly sensitive to nickel. In absence of nickel or cobalt RcnR binds to the operator and inhibits the nickel responsive promoter. With Ni(II)-ions present the repression of the promoter RcnA will be reversed, because the repressor RcnR binds nickel-ions and cannot attach to the DNA. For our biosensor we construct the part ( BBa_K1758353 by using the basic construction showed in . For this part we used the repressor RcnR under control of a constitutive promoter ( BBa_K1758350 ) and the nickel specific promoter RcnA with a 5’UTR in front of sfGFP ( BBa_K1758352 ) as reporter protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 509