Difference between revisions of "Part:BBa K1766000"

(→‎MicF)
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
−
== '''MicF''' ==
+
__NOTOC__
 +
<partinfo>BBa_K1766000 short</partinfo>
  
  
−
MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene  in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and  promote degradation.
+
MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene  in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and  promote degradation [1].
  
−
BBa_K1766000 can be used together with BBa_K1766001 (MicF Target) to regulate protein expression.
+
BBa_K1766000 is intended to be used together with BBa_K1766001 (MicF Target) to regulate protein expression.
 +
 
 +
The BioBrick has been sequenced and confirmed as the correct construct. Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.
  
  
Line 10: Line 13:
 
<span class='h3bb'>'''Sequence and Features'''</span>
 
<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K1766000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1766000 SequenceAndFeatures</partinfo>
 +
 +
 +
'''References'''
 +
 +
[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12

Latest revision as of 23:28, 17 September 2015

MicF non-coding regulatory RNA


MicF is a non-coding RNA which regulates expression of the ompF (outer membrane porin F) gene in E. coli. MicF binds to the 5’-UTR of ompF mRNA, to prevent translation and promote degradation [1].

BBa_K1766000 is intended to be used together with BBa_K1766001 (MicF Target) to regulate protein expression.

The BioBrick has been sequenced and confirmed as the correct construct. Due to time constraints iGEM Stockholm 2015 were unable to caracterize this part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12