Difference between revisions of "Part:BBa K1707026"

(Usage and Biology)
(Usage and Biology)
 
(2 intermediate revisions by the same user not shown)
Line 8: Line 8:
 
We characterized this part using fluorescence microscopy.
 
We characterized this part using fluorescence microscopy.
  
Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170726
+
'''Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170726'''
  
[[File:Paris_Saclay_K1707026_w1POL.png|400px]]
+
[[File:Paris_Saclay_K1707026_w1POL.png|300px]]
 
Differential interference contrast
 
Differential interference contrast
[[File:Paris_Saclay_K1707026_w2Lumencor CFP 434-17.png|400px]]
+
 
excitation with led 437 nm/17nm (CFP), 100 ms of exposure
+
[[File:Paris_Saclay_K1707026_w2Lumencor CFP 434-17.png|300px]]
 +
Excitation with led 437 nm/17nm (CFP), 100 ms of exposure
  
  

Latest revision as of 23:28, 17 September 2015

CFP-ssRA under the control of J23101

This part was constructed to have a positive control for the expression of CFP-ssRA (under the control of a strong promoter).


Usage and Biology

We characterized this part using fluorescence microscopy.

Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170726

Paris Saclay K1707026 w1POL.png Differential interference contrast

Paris Saclay K1707026 w2Lumencor CFP 434-17.png Excitation with led 437 nm/17nm (CFP), 100 ms of exposure


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]