Difference between revisions of "Part:BBa K1707026"

Latest revision as of 23:28, 17 September 2015

CFP-ssRA under the control of J23101

This part was constructed to have a positive control for the expression of CFP-ssRA (under the control of a strong promoter).

We characterized this part using fluorescence microscopy.

Figure 1: Fluorescence microscopy of E. coli DH5alpha bearing K170726

Differential interference contrast

Excitation with led 437 nm/17nm (CFP), 100 ms of exposure

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1707026 short</partinfo>
@@ Line 5: / Line 4: @@
 This part was constructed to have a positive control for the expression  of CFP-ssRA (under the control of a strong promoter).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+We characterized this part using fluorescence microscopy.
+'''Figure 1: Fluorescence microscopy of ''E. coli'' DH5alpha bearing K170726'''
+[[File:Paris_Saclay_K1707026_w1POL.png|300px]]
+Differential interference contrast
+[[File:Paris_Saclay_K1707026_w2Lumencor CFP 434-17.png|300px]]
+Excitation with led 437 nm/17nm (CFP), 100 ms of exposure
 <!-- -->